Alternatively, if you harvest those crystals, wash them well in growth solution and run SDS PAGE you may also get an answer.
Vaheh
-----Original Message-----
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Tim Gruene
Sent: Friday, May 04, 2012 8:53 AM
To: [log in to unmask]
Subject: Re: [ccp4bb] detergent or protein
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Dear Anita,
you cannot have a definite answer until to shoot the crystal and
determine its cell.
Regards,
Tim
On 05/04/12 14:48, anita p wrote:
> Hi All, I would like to have your expert advice on crystals. I am
> using detergents as 5% (w/v) of DDM 0.4ul in a 4ul (protein +
> condition + detergent). The precipitant is 28% peg 20K After 1 day
> I am able to see little plates of irregular shape .
>
> I am able to see some needles if I change into MEGA-10.
>
> I am able to see some hexagonal 3D crystals when I change the
> precipitant to Ammonium sulphate and the detergent to Anapoe-C10E6
>
> Is there a possibility to check whether this crystals are protein
> or of detergent, even before shooting it ? Does the control drop
> test help ??
>
> awaiting for feedback Anita
>
- --
- --
Dr Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
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