Hi Anita,
One question that comes up immediately is why you are using DDM @ 5% concentration. If it's a typo, understandable. I figure most of us use DDM at 0.05-0.1% concentration, and I've used as low as 0.015%.
Also, as others have pointed out, until you shoot it, you don't know. But if it's hexaganol with very smooth/non-sharp edges, many of us have seen it using DDM.
-john
On May 4, 2012, at 7:48 AM, anita p wrote:
> Hi All,
> I would like to have your expert advice on crystals.
> I am using detergents as 5% (w/v) of DDM 0.4ul in a 4ul (protein + condition + detergent). The precipitant is 28% peg 20K
> After 1 day I am able to see little plates of irregular shape .
>
> I am able to see some needles if I change into MEGA-10.
>
> I am able to see some hexagonal 3D crystals when I change the precipitant to Ammonium sulphate and the detergent to Anapoe-C10E6
>
> Is there a possibility to check whether this crystals are protein or of detergent, even before shooting it ?
> Does the control drop test help ??
>
> awaiting for feedback
> Anita
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