-----BEGIN PGP SIGNED MESSAGE-----
Hash: SHA1
Dear Anita,
you cannot have a definite answer until to shoot the crystal and
determine its cell.
Regards,
Tim
On 05/04/12 14:48, anita p wrote:
> Hi All, I would like to have your expert advice on crystals. I am
> using detergents as 5% (w/v) of DDM 0.4ul in a 4ul (protein +
> condition + detergent). The precipitant is 28% peg 20K After 1 day
> I am able to see little plates of irregular shape .
>
> I am able to see some needles if I change into MEGA-10.
>
> I am able to see some hexagonal 3D crystals when I change the
> precipitant to Ammonium sulphate and the detergent to Anapoe-C10E6
>
> Is there a possibility to check whether this crystals are protein
> or of detergent, even before shooting it ? Does the control drop
> test help ??
>
> awaiting for feedback Anita
>
- --
- --
Dr Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
GPG Key ID = A46BEE1A
-----BEGIN PGP SIGNATURE-----
Version: GnuPG v1.4.12 (GNU/Linux)
Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/
iD8DBQFPo9EnUxlJ7aRr7hoRAo1BAJ4ryuYgYLpZ4QKzJr1hP4AEwAU9SACfZ8OM
V62rCE19xVxyQbyRQ69mEoc=
=ol53
-----END PGP SIGNATURE-----
|