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You got help, but you'll have to do the reading yourself.
Cheers,
Tim
On 05/05/12 08:53, ruby singh wrote:
> On Sat, May 5, 2012 at 12:17 PM, ruby singh
> <[log in to unmask]> wrote:
>
>> Sir, i m sorry for not giving full details of protein. its plant
>> lectin protein on which im working currently. its sequence
>> information is very little. crystallization has become near to
>> impossible. the protein is a tetramer( of 28KDa). its
>> purification is very simple...but for crystallization i tried
>> many things nt working though(Hampton Reserach-pegion and
>> index/CSS1/JCSG etc..). need some help. the protein
>> conecentration used was 30mg/ml stock . most of the
>> crystallization condition shows precipitation. the set up was put
>> at 20C. the protein is very stable in MilliQ water upto 100mg/ml
>> concentration with no precipitation.
>>
> the protein doesnt aggregate..doesnt loose activity even after
> boiling at 80C.
>
> getting its crystal is important for my thesis. so need help.
>
- --
- --
Dr Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
GPG Key ID = A46BEE1A
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