The guidelines are in Arch Pathol Lab Med 2000;124:71-81.
Mario D'Costa Ph.D. FCACB
Director of Clinical Chemistry and Core Lab
St. Joseph's Health Centre
30 The Queensway, Toronto
On, Canada, M6R 1B5
Tel: 416-530-6263
Fax: 416-530-6559
email: [log in to unmask]
>>> <[log in to unmask]> 12/06/01 10:59AM >>>
I know there is a set of guidelines published not so long ago but I cannot
seem to recall the source at this moment. However, you might find the
article by Kern, Kron & Hiesche in Clin Diagn. Lab. Immunol. Vol 7 (1) 2000,
pg 72-78 to be useful.
Godfrey Moses
Godfrey Moses, BSc., MSc., PhD, FCACB
Clinical Biochemist
Medical Support,
MDS Inc.
[log in to unmask]
416-675-6777 ext 2363
-----Original Message-----
From: Marcio Pacheco de Andrade [mailto:[log in to unmask]]
Sent: Thursday, December 06, 2001 5:05 AM
To: [log in to unmask]
Subject: Antinuclear antibodies
Dear Members
Maybe You can help me....
We have a patient woman, 52 yo, veterinary, with no medication, with
pain in fingers, knees, elbows and back.
The GP requisite a ANA. We perform the ANA with a BION kit (Hep-2) and
the results was a small fluorescence only in a 1:40 dilution, 1:160, 1:640
show a negative results. We repeated this test 2 days after and have same
results.
The patient still feel pain and we send the sample to a reference lab
and the result was : reagent until 1:160 dilution, fine punctate pattern in
Hep-2 cels.
My questions are:
1) Why so much difference between the kits?
2) What is the correct conduct to take in this case?(repeat test,
indicate new antigen like SSa and SSb or Scl-70)
3) Where can I get a guideline to ANA test?
Thanks in advance
Márcio Pacheco de Andrade
[log in to unmask]
Laboratorio Pacheco Ltda
Lages - SC - Brazil
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