We use a commercial product called Lipoclear (StatSpin Technology, 85 Morse
St., Norwood, MA 02062; Phone 617-551-0100 or 800-782-8774). In the package
insert, the product is described as non-ionic non-toxic polymer. We did
some studies in house (for electrolytes, BUN, Cr and enzymes) and found the
product quite satisfactory.
Hopefully, this is helpful.
Uttam Garg, Ph.D.
Director, Clinical Chemistry & Toxicology
Children's Mercy Hospital
Kansas City, MO 64108
Phone: 816-234-3803
Fax: 816-234-3794
> -----Original Message-----
> From: Lars Eikvar [SMTP:[log in to unmask]]
> Sent: Wednesday, April 07, 1999 3:12 AM
> To: [log in to unmask]
> Subject: separation of lipids from strongly lipemic samples
>
> Dear colleagues
>
> Strongly lipemic samples can cause significant interference with several
> routine measurements in clinical chemistry. There is always possibility of
> ultra centrifugation, but this is not an option for STAT samples. In our
> laboratory (and in several other Norwegian labs), we have been applying a
> technique of extracting the samples with 1,1,2-trichloro
> -1,2,2-trifluoroethane (formerly marketed as Frigen, Hoecst). This removes
> lipids and lipoproteins, and we have found that the procedure does not
> interfere significantly with most of our assays.
>
> I have only found a few references in Medline mentioning this technique.
> Most of our investigations were performed 15-20 years ago, and we never
> came around to write about this ourselves.
>
> This component is a CFC (and thus ozone layer depleting), and its use is
> regulated by the Montreal convention. There is a paragraph exempting the
> use of these components in laboratories, but we still have to apply for a
> permission to use this chemical each time we order a few kg. Our yearly
> consumption is approx. 1-2 kg.
>
> There is now talk about removing this exemption from the list. Thus, we
> may
> now have to look for alternative procedures for removing lipids from such
> samples.
>
>
> I would greatly appreciate any views on this matter, especially
> suggestions
> for alternative procedures.
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