 |
 |
We use a commercial product called Lipoclear (StatSpin Technology, 85 Morse St., Norwood, MA 02062; Phone 617-551-0100 or 800-782-8774). In the package insert, the product is described as non-ionic non-toxic polymer. We did some studies in house (for electrolytes, BUN, Cr and enzymes) and found the product quite satisfactory. Hopefully, this is helpful. Uttam Garg, Ph.D. Director, Clinical Chemistry & Toxicology Children's Mercy Hospital Kansas City, MO 64108 Phone: 816-234-3803 Fax: 816-234-3794 > -----Original Message----- > From: Lars Eikvar [SMTP:[log in to unmask]] > Sent: Wednesday, April 07, 1999 3:12 AM > To: [log in to unmask] > Subject: separation of lipids from strongly lipemic samples > > Dear colleagues > > Strongly lipemic samples can cause significant interference with several > routine measurements in clinical chemistry. There is always possibility of > ultra centrifugation, but this is not an option for STAT samples. In our > laboratory (and in several other Norwegian labs), we have been applying a > technique of extracting the samples with 1,1,2-trichloro > -1,2,2-trifluoroethane (formerly marketed as Frigen, Hoecst). This removes > lipids and lipoproteins, and we have found that the procedure does not > interfere significantly with most of our assays. > > I have only found a few references in Medline mentioning this technique. > Most of our investigations were performed 15-20 years ago, and we never > came around to write about this ourselves. > > This component is a CFC (and thus ozone layer depleting), and its use is > regulated by the Montreal convention. There is a paragraph exempting the > use of these components in laboratories, but we still have to apply for a > permission to use this chemical each time we order a few kg. Our yearly > consumption is approx. 1-2 kg. > > There is now talk about removing this exemption from the list. Thus, we > may > now have to look for alternative procedures for removing lipids from such > samples. > > > I would greatly appreciate any views on this matter, especially > suggestions > for alternative procedures. %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%