Fig. 5 in this paper also has some data, including the effect of the
phase plate:
https://www.ncbi.nlm.nih.gov/pubmed/27235783
But as earlier noted, it can be expected to be sample dependent
(alignment, ice thickness, etc). So for "non-ribosome" objects you may
need to recalibrate the scale.
Cheers,
Lars
On 2019-08-09 07:54, Bruno KLAHOLZ wrote:
> Dear Reza,
>
> while not entirely answering your question, here is a paper from us
> related with the resolution you can get from a certain amount of
> particles using different settings with single versus dual axis
> tomography after a given number of refinement cycles during sub-tomogram
> averaging:
>
> https://www.ncbi.nlm.nih.gov/pubmed/23376404
>
> Best,
>
> Bruno
>
> *From:*Collaborative Computational Project in Electron cryo-Microscopy
> [mailto:[log in to unmask]] *On Behalf Of *Reza Khayat
> *Sent:* 09 August 2019 00:24
> *To:* [log in to unmask]
> *Subject:* [ccpem] Subtomogram averaging and resolution
>
> Hi,
>
> Is there a publication that compares the number of
> subtomograms/particles necessary to reach particular resolutions? I
> appreciate the help.
>
> Best wishes,
> Reza
>
> Reza Khayat, PhD
>
> Assistant Professor
>
> City College of New York
>
> Department of Chemistry
>
> New York, NY 10031
>
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--
Lars-Anders Carlson
Assistant Professor
Dept of Medical Biochemistry and Biophysics
Wallenberg Centre for Molecular Medicine
Umeå University
901 87 Umeå, Sweden
http://www.carlsonlab.se/
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