FYI: we typically use 100-200 classes, and never more than 300 classes.
I think 1,000 classes would be a waste of your resources. Try 200 first.
HTH, S
On 12/13/2013 12:13 PM, Ludovic Renault wrote:
>
> Thanks for your reply.
>
> We are at 3 x binned pixel size. It's a close call. I'll try to
> increase the
>
> threads number again and see if that helps. I was trying 400-1,000
> classes.
>
>
> To make it work, I was thinking I could use the 'rescale' option at the
>
> pre-processing extraction step, clean up my data and then do the final 3d
>
> refinement on the original data. Is that possible?
>
> L
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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