Hi Joel,
I can't really tell from this information. Until 40A the two
half-reconstructions are combined to prevent orientational differences
between them. Thereby, the minimum resolution will always be close to
40A. If you think your maps have more resolution, something must have
gone wrong. Do the masks look okay?
HTH, S
On 12/22/2013 09:44 PM, J. Meyerson wrote:
> Hi Sjors,
> I processed three 3D classes using the refinement routine following by
> post-processing. When I examine the three "postprocess_run?_ct??.star"
> file headers, I see that all three resolutions are the same
> (37.493334). Additionally, this resolution is lower than what was
> given prior to post-processing the maps. Can you help me understand
> why this is happening? I've provided the three headers below.
>
> Thanks,
> Joel
>
>
> # RELION postprocess
> # --i Refine3D/run1_ct10 --o Refine3D/postprocess_run1_ct10 --angpix
> 1.406 --inimask_threshold 0.016 --auto_mask --skip_fsc_weighting
>
> data_general
>
> _rlnFinalResolution 37.493334
> _rlnBfactorUsedForSharpening 0.000000
>
>
>
>
> # RELION postprocess
> # --i Refine3D/run2_ct14 --o Refine3D/postprocess_run2_ct14 --angpix
> 1.406 --inimask_threshold 0.013 --auto_mask --skip_fsc_weighting
>
> data_general
>
> _rlnFinalResolution 37.493334
> _rlnBfactorUsedForSharpening 0.000000
>
>
>
>
> # RELION postprocess
> # --i Refine3D/run3_ct11 --o Refine3D/postprocess_run3_ct11 --angpix
> 1.406 --inimask_threshold 0.02 --auto_mask --skip_fsc_weighting
> --low_pass 20
>
> data_general
>
> _rlnFinalResolution 37.493334
> _rlnBfactorUsedForSharpening 0.000000
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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