David,
Did you normalise your particles in relion? At least for one particle the
normalisation is very far off.
HTH, S
> Dear Sjors,
>
> I started and a classification of a dataset. The first 2 iterations
> worked quite nicely, however in iteration 3 already at the start I get
> strange outputs:
>
> WARNING: Option --dont_combine_weights_via_disc is not a valid argument
> 7/ 7 sec
> ............................................................~~(,_,">
> 000/??? sec ~~(,_,">
> [oo] mymodel.current_size= 48 mymodel.ori_size= 256 part_id= 28416
> DIRECT_A2D_ELEM(exp_metadata, my_image_no, METADATA_NORM)= 16.9222
> mymodel.avg_norm_correction= 0.720389
> exp_wsum_norm_correction[ipart]= 6.00675
> old_norm_correction= 4.88227
> wsum_model.avg_norm_correction= 26.3485
> group_id= 0 mymodel.scale_correction[group_id]= 1
> mymodel.sigma2_noise[group_id]=
> 0.91543
> 0.6284
> 0.42518
> 0.26224
> 0.21422
> 0.19368
> 0.19689
> 0.17972
>
> this goes on. Morover it wrote debug_class00x.mrc files as well as a
> debug_model.star file.
>
> Do you have any clue what could have gone wrong?
>
> cheers
>
> David
>
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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