Hi Joe,
We have a 32-head Honeybee robot which sets up a 96-well plate with a
single drop per well in ~6 minutes and 3 drops per well at ~9 minutes. A
96-head phoenix or hummingbird-like system is likely going to be faster,
but not by that much. Our robot has its own humidified cabinet and as
long as you keep evaporation under control I don't think set-up speed,
within reason, is that important.
Bart
JOE CRYSTAL wrote:
> Hi,
>
>
> Does anyone have information about how long it takes to set up a 96-well
> tray for the crystallization robots available? Besides cost per tray
> and maintenance cost, another important feature we consider is the time
> for setting up a 96-well tray. It is an important factor since we are
> talking about sub-microliter drops.
>
>
> Best,
>
>
> Joe
>
> On Fri, Jan 18, 2008 at 12:28 PM, Lisa A Nagy <[log in to unmask]
> <mailto:[log in to unmask]>> wrote:
>
> Al's Oil on the plates:
> What a nightmare!!!!!!!
> The oil creeps up the plate and over the sides. It dissolves adhesives.
> It makes me say bad words in multiple languages.
> Bigger drops + no oil = fewer bad words.
>
> Lisa
> --
> Lisa A. Nagy, Ph.D.
> University of Alabama-Birmingham
> [log in to unmask] <mailto:[log in to unmask]>
>
> -----Original Message-----
> From: CCP4 bulletin board [mailto:[log in to unmask]
> <mailto:[log in to unmask]>] On Behalf Of
> Patrick Shaw Stewart
> Sent: Friday, January 18, 2008 2:20 AM
> To: [log in to unmask] <mailto:[log in to unmask]>
> Subject: [ccp4bb] Fwd: [ccp4bb] crystallisation robot
>
> One thing that people often overlook is that quite a lot of protein
> can be lost by denaturation on the surface of the drop. This is more
> significant for smaller drops. Two suggestions: (1) increase the
> proportion of protein in the - technical term - teeny drop to say two
> thirds and (2) cover the drops with oil eg Al's oils
> (silicone/paraffin). You still get vapor diffusion though the oil ,
> and you'd like to slow up equilibration. of course (2) slows up the
> robotics a little, but both should be trivial to set up..
>
>
--
=============================================================================
Bart Hazes (Assistant Professor)
Dept. of Medical Microbiology & Immunology
University of Alberta
1-15 Medical Sciences Building
Edmonton, Alberta
Canada, T6G 2H7
phone: 1-780-492-0042
fax: 1-780-492-7521
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