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Hi Joe, We have a 32-head Honeybee robot which sets up a 96-well plate with a single drop per well in ~6 minutes and 3 drops per well at ~9 minutes. A 96-head phoenix or hummingbird-like system is likely going to be faster, but not by that much. Our robot has its own humidified cabinet and as long as you keep evaporation under control I don't think set-up speed, within reason, is that important. Bart JOE CRYSTAL wrote: > Hi, > > > Does anyone have information about how long it takes to set up a 96-well > tray for the crystallization robots available? Besides cost per tray > and maintenance cost, another important feature we consider is the time > for setting up a 96-well tray. It is an important factor since we are > talking about sub-microliter drops. > > > Best, > > > Joe > > On Fri, Jan 18, 2008 at 12:28 PM, Lisa A Nagy <[log in to unmask] > <mailto:[log in to unmask]>> wrote: > > Al's Oil on the plates: > What a nightmare!!!!!!! > The oil creeps up the plate and over the sides. It dissolves adhesives. > It makes me say bad words in multiple languages. > Bigger drops + no oil = fewer bad words. > > Lisa > -- > Lisa A. Nagy, Ph.D. > University of Alabama-Birmingham > [log in to unmask] <mailto:[log in to unmask]> > > -----Original Message----- > From: CCP4 bulletin board [mailto:[log in to unmask] > <mailto:[log in to unmask]>] On Behalf Of > Patrick Shaw Stewart > Sent: Friday, January 18, 2008 2:20 AM > To: [log in to unmask] <mailto:[log in to unmask]> > Subject: [ccp4bb] Fwd: [ccp4bb] crystallisation robot > > One thing that people often overlook is that quite a lot of protein > can be lost by denaturation on the surface of the drop. This is more > significant for smaller drops. Two suggestions: (1) increase the > proportion of protein in the - technical term - teeny drop to say two > thirds and (2) cover the drops with oil eg Al's oils > (silicone/paraffin). You still get vapor diffusion though the oil , > and you'd like to slow up equilibration. of course (2) slows up the > robotics a little, but both should be trivial to set up.. > > -- ============================================================================= Bart Hazes (Assistant Professor) Dept. of Medical Microbiology & Immunology University of Alberta 1-15 Medical Sciences Building Edmonton, Alberta Canada, T6G 2H7 phone: 1-780-492-0042 fax: 1-780-492-7521 =============================================================================