CK has mol. wt. of approx 40,000 and so will appear in the urine particularly in a patient following surgery or acute MI at least partly because of increased vascular permeability. Activities of much larger enzymes such as NAG also increase following trauma. CKBB is the predominant isoenzyne in renal tissue so CKMB activity in urine would be of serum origin. A word of caution however. In my experience it is not possible to take a serum enzyme assay and use it for urine without considering the presence of enzyme inhibitors or even activators in urine. Also, just freezing urine (-20 oC) can result in loss of enzyme activity as can prolonged storage at 4 oC. Sorry that I don't know of anyone assaying CK in urine.
Mike
Dr. Mike Bosomworth
Operations Manager for Pathology
Principal Biochemist
Tel. 0113 2064299 Fax 0113 2065971
Mobile 07789 174344
Please visit our web-site at www.leedsteachinghospitals.com
>>> John Meek <[log in to unmask]> 20/05/2003 12:02:57 >>>
A clinical colleague would like to know (normal) reference values for CK
and CKMB in urine. I imagine that there is very little in normal urine,
but maybe someone has had cause to look at trace amounts. If so, he
would then like to know how to get it measured (I suspect in a
post-cardiac surgery situation)!
John Meek
Clinical Chemistry
Hammersmith Hospital, London W12.
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