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CK has mol. wt. of approx 40,000 and so will appear in the urine particularly in a patient following surgery or acute MI at least partly because of increased vascular permeability. Activities of much larger enzymes such as NAG also increase following trauma. CKBB is the predominant isoenzyne in renal tissue so CKMB activity in urine would be of serum origin. A word of caution however. In my experience it is not possible to take a serum enzyme assay and use it for urine without considering the presence of enzyme inhibitors or even activators in urine. Also, just freezing urine (-20 oC) can result in loss of enzyme activity as can prolonged storage at 4 oC. Sorry that I don't know of anyone assaying CK in urine. Mike Dr. Mike Bosomworth Operations Manager for Pathology Principal Biochemist Tel. 0113 2064299 Fax 0113 2065971 Mobile 07789 174344 Please visit our web-site at www.leedsteachinghospitals.com >>> John Meek <[log in to unmask]> 20/05/2003 12:02:57 >>> A clinical colleague would like to know (normal) reference values for CK and CKMB in urine. I imagine that there is very little in normal urine, but maybe someone has had cause to look at trace amounts. If so, he would then like to know how to get it measured (I suspect in a post-cardiac surgery situation)! John Meek Clinical Chemistry Hammersmith Hospital, London W12. * ** Confidentiality Notice The contents of this e-mail are confidential to the ordinary user of the e-mail address to which it is addressed and may also be privileged. If you are not the addressee of this e-mail you may not copy, forward, disclose or otherwise use it in any form whatsoever. If you have received this e-mail in error please telephone the Hammersmith Hospitals NHS Trust IT department on 020 8746 5555. Please also delete the message from your computer. ** * ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/ ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/