I note both CK tests are reported in units. The activity based assay for CKMB works by inhibiting the CK-M subunit, measuring B and then multiplying by 2 to get a CKMB activity. Therefore if you have a lot of CK-BB, you get wierd results - This sample could be the results of having 725 UNits of the total CK activity due to BB subunit ? a brain injury???
TIM
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Prof. T. Reynolds,
Clinical Chemistry Dept,
Queens Hospital,
Belvedere Rd.,
Burton-on-Trent,
STAFFS,
DE13 0RB.
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Tel: +44 (0)1283 511511 ext. 4035
Fax: +44 (0)1283 593064
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-----Original Message-----
From: c=GB;a=NHS;p=NHS NATIONAL INT;dda:RFC-822=acb-clin-chem-gen-request(a)mailbase.ac.uk;
Sent: Thursday, October 26, 2000 2:37 PM
To: c=GB;a=NHS;p=NHS NATIONAL INT;dda:RFC-822=acb-clin-chem-gen(a)mailbase.ac.uk;
Subject: CKMB problem
A recent request for CKMB revealed a total CK of 778 IU/L and a CKMB of 1449
IU/L (both were measured using Roche reagents). Other tests and controls in the
batch did not reveal any such anomalies.
I have a vague recollection of this sort of effect possibly being caused by
binding of the enzyme to an immunoglobulin.
Any other ideas?
Dr D G Williams
FRCPath
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