Dear Arthur,
I know from a collaboration, that solving cryoEM structures of
proteins in nanodiscs in principle works fine. Our collaborator was
able to solve the structure of a membrane transporter at close to 3
Angstrom.
However, I think the important aspect in your case is, whether the
protein occupies a distinct orientation inside the nanodisc, or if it
will be randomly oriented. Given the last, the nanodisc will most
likely not help at all, since you will align all the particles
according to the mass of the nanodisc, and your protein will then be
missaligned. To bypass this problem, one would need to do masked
refinements/classifications including only the inner part of the
nanodisc and you would end up with the original problem.
Best,
Julian
Zitat von sunyeping <[log in to unmask]>:
> Dear everyone,
>
> I would like to try to solve the structure of a small protein with a
> molecular weight of about 40 kd. Obviously it is too small for the
> current single-particle cryo-EM techniques. But I think if it is
> inserted into a nanodisc, it means to add ~200 kd to the small
> protein. So, nanodisc can be used to solve cryo-EM structure, isn't
> it? Is there other problems in solving the small protein structure
> in this way?
>
> Best regard.
> Arthur
>
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--
Julian Reitz, Dipl.-Biochem.
J.W. Goethe Universität Frankfurt am Main
BMLS - Buchmann Institut for Molecular Life Sciences
Frangakis Group
Max-von-Laue-Str. 15 Raum 1.658, D-60438
Tel: +49 (0)69 798-46429
e-mail: [log in to unmask]
http://fcem.uni-frankfurt.de/em
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