I used NaF in the precipitant thrice for three different proteins to be crystallized & each time I got crystals and they were all salt crystals ! I thought low solubility of NaF causes this quick crystallization of salt.
Recently I got crystals of a protein at 20 degree grown in gradient concentration of AmSO4 as precipitant.In some cover-slips I got precipitate initially but I kept the tray at RT for mounting at home-source & after some time I saw small crystals appeared in those drops which contained precipitate initially.Though the small crystals never grew enough for mounting. Some drops were used for seeding,small crystals appeared but they dissolved/disappeared at RT, again reappeared after several weeks as larger one.
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