Have not calculated it myself , but I have been led to believe that 50ul of
whole blood added to 2ml of CSF increases concentration of protein by to 4
times in normal CSF. Therefore even a small amount of blood will invalidate
the results. We therefore reject any samples with traces of blood for
protein analysis.
Our clinicians to achieve serial sampling.
Do not have details on Glucose, but fluoride oxalate appears to increase
apparent protein levels if used for protein analysis.
Tim Hogan
Basildon.
-----Original Message-----
From: Peter Stromberg [mailto:[log in to unmask]]
Sent: 27 February 2003 12:11
To: [log in to unmask]
Subject: CSF ANALYSIS
Does anyone have any hard data or/and references concerning the following.
A>The stability of glucose in CSF samples collected into fluoride-oxalate
tubes or tubes without preservative..Does anyone have a reference with lots
of hard data in it?
B>In CSF samples that contain red cells ,in the broadest sense ,what
criteria to listeners use to determine when samples are unsuitable for
protein assay?Do people hold to sample against their white coat and dont do
protein on the pink ones are are there more objective ways of doing it?
C>The proposed guidelines published on the Web by Beetham et al speak of
serial samples being collected.Does this happen in practice ?Is this
achievable by the average operator?
D>My impression is that we should stop using the word xanthochromia.If
subarachnoid haemorrhage is on the differential diagnosis a scan should be
done by someone who knows how.Xanthochromia has a specific meaning for
clinicians and since external QC suggests we cant reliably assess it perhaps
we should use many of the other useful English words.Is this reasonable?
------ACB discussion List Information--------
This is an open discussion list for the academic and clinical
community working in clinical biochemistry.
Please note, archived messages are public and can be viewed
via the internet. Views expressed are those of the individual and
they are responsible for all message content.
ACB Web Site
http://www.acb.org.uk
List Archives
http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html
List Instructions (How to leave etc.)
http://www.jiscmail.ac.uk/
**********************************************************************
This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed. If you have received this email in error please notify
the system manager at mailto:[log in to unmask]
Visit our website on www.basildonandthurrock.nhs.uk
<http://www.basildonandthurrock.nhs.uk>
This footnote also confirms that this email message has been swept by
MIMEsweeper for the presence of computer viruses.
www.mimesweeper.com
**********************************************************************
------ACB discussion List Information--------
This is an open discussion list for the academic and clinical
community working in clinical biochemistry.
Please note, archived messages are public and can be viewed
via the internet. Views expressed are those of the individual and
they are responsible for all message content.
ACB Web Site
http://www.acb.org.uk
List Archives
http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html
List Instructions (How to leave etc.)
http://www.jiscmail.ac.uk/
|