Although, in theory measured total iron binding capacity (TIBC) by
alumina or other resins absorption/iron measurement should correlate
well with calculated TIBC from transferrin, practically how good the
correlation is? It seems that most of the labs use absorption methods
for TIBC, despite of large sample volume needed for the assay and more
laborious assay. Literature is quite variable on the ratios of TIBC and
transferrin. We got the following data (measured TIBC on X-axis;
calculated TIBC on Y-axis).
slope 1.22
Intercept -19.62
RSQ 0.922
r 0.962
TIBC Range 205-484 ug/dL
Calculated values are ~20% higher than measure values (to get TIBC in
ug/dL, values of trasferrin in mg/dL were multiplied by 1.43).
How is the experience of other people in switching measured TIBC to
calculated TIBC from transferrin? Thanks for comments/suggestions.
Uttam Garg, Ph.D.
Director of Clinical Chemistry
and Toxicology Laboratories
Children's Mercy Hospital
Kansas City, MO 64108
Phone: 816-234-3803
Fax: 816-234-3794
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