I have a question for the chromatographers in the mailbase-
What affect will a small leak at the outlet valve on the pump head have on
retention times in a gradient method? I have heard that a leak in an HPLC system
can lead to a disproportionate loss of organic modifier (e.g. MeCN) through the
leak. Is this true? And if so, can this phenomenon lead to longer retention
times in a gradient run?
Any comments and suggestions would be appreciated.
Thanks
Tim
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