Hi,
Last frames have less information, so fitting is more difficult.
There can also be a shutter synchronization problem.
But we usually don't care, because high resolution signal is already
dead there.
You can manually change B factors if you really want:
(1) edit bfactors.star
(2) delete shiny.star and *shiny.mrcs
(3) press 'continue.
The program will recombine frames using the modified B factors.
Best regards,
Takanori Nakane
On 2019/02/12 8:05, Daniel Larsson wrote:
> Hi all,
>
> How do you evaluate which resolution limits to use during Bayesian polish. Are there any good general rule of thumb? In the old particle polish, individual Guinier Plots were printed for each movie frame, which I think was very useful.
>
> Here is an example of a strange result that I got when using the default settings (min = 20, max = -1), with a spike in the last frame.
>
> Regards,
> Daniel
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