Dear Sjors and all,
I have 2 questions:
1) I did a reconstruction by using movie frames 4 to 20. I now want to
see if I get to a better resolution by using e.g. frames 3 to 17:
I did run the corresponding motioncor2 followed by Ctfrun (don't know
whether this is necessary or the values will largely remain the same).
What is the best way to extract these new particle images by using the
old run_data.star file from the refinement run on frames 4 - 20? In
this file I see references to the previous extraction job and to the
micrographs. What do I need to change?
2) It appears to me that "relion_mask_create" has changed in relion-2.1
beta 0: calling it from command line gives:
ERROR:
Either provide --i to apply a mask, OR --create_from to create a new mask
File: /opt/relion/src/apps/mask_create.cpp line: 66
and "relion_mask_create --create_from" gives:
ERROR:
Either provide --i to apply a mask, OR --create_from to create a new mask
File: /opt/relion/src/apps/mask_create.cpp line: 66
instead of listing the parameters. Is this the intended behavior?
Thanks a lot for hints, Dieter
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Dieter Blaas,
Max F. Perutz Laboratories
Medical University of Vienna,
Inst. Med. Biochem., Vienna Biocenter (VBC),
Dr. Bohr Gasse 9/3,
A-1030 Vienna, Austria,
Tel: 0043 1 4277 61630,
Fax: 0043 1 4277 9616,
e-mail: [log in to unmask]
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