Hi Matthew,
William seems to want to increase the x-ray intensity gradually during the experiment by augmenting the transmission gradually. It that possible? If so, would it be advisable?
Mark J van Raaij
Dpto de Estructura de Macromoleculas
Centro Nacional de Biotecnologia - CSIC
c/Darwin 3
E-28049 Madrid, Spain
tel. (+34) 91 585 4616
http://www.cnb.csic.es/~mjvanraaij
On 24 Apr 2015, at 12:45, Matthew BOWLER wrote:
> Dear William,
> at the ESRF we have a Helical characterisation workflow that will calculate a strategy based on the observed diffraction patterns and the distance between 2 points selected on a crystal, best wishes, Matt.
>
>
>
> On 2015-04-24 12:00, William Chao wrote:
>> Dear all,
>>
>> Does anyone have experience in maximising anomalous signal during a
>> helical/line scan? Most (all?) automated data collection strategies in
>> synchrotrons seem to work on a single point of a crystal but don't
>> give strategies for line scan. Would it be advisable to increase the
>> recommended transmission as radiation is spread along the crystal? If
>> the strategy suggests us to use 10% transmission (100% = 2x10^11 ph/s)
>> for a 360-degree collection on a single point, to what amount should I
>> increase say along a 200 micron scan with a 20 micron beam (crystal
>> dimension 200x100x20)?
>>
>> Thank you very much in advance for the suggestions!
>>
>> William
>> ---
>>
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>
> --
> Matthew Bowler
> Synchrotron Diffraction Group
> European Molecular Biology Laboratory
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