Dear William,
at the ESRF we have a Helical characterisation workflow that will
calculate a strategy based on the observed diffraction patterns and the
distance between 2 points selected on a crystal, best wishes, Matt.
On 2015-04-24 12:00, William Chao wrote:
> Dear all,
>
> Does anyone have experience in maximising anomalous signal during a
> helical/line scan? Most (all?) automated data collection strategies
> in
> synchrotrons seem to work on a single point of a crystal but don't
> give strategies for line scan. Would it be advisable to increase the
> recommended transmission as radiation is spread along the crystal? If
> the strategy suggests us to use 10% transmission (100% = 2x10^11
> ph/s)
> for a 360-degree collection on a single point, to what amount should
> I
> increase say along a 200 micron scan with a 20 micron beam (crystal
> dimension 200x100x20)?
>
> Thank you very much in advance for the suggestions!
>
> William
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>
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--
Matthew Bowler
Synchrotron Diffraction Group
European Molecular Biology Laboratory
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