Dear Sjors,
I ran a 3D-classification into 2 classes until iteration 25 without
problems. I then extracted the particle images corresponding to the
respective classes, made CTF groups containing not less than 20
particles per group and ran 3D-Refinements on both classes without
problems up to iteration 16 for one and iteraction 6 for the other
class. I then received the error message:
Expectation iteration 16
0.08/2.18 min ..~~(,_,"> exp_thisparticle_sumweight=
nan [$
exp_part_id= 2039exp_iimage=1
group_id= 61 mymodel.scale_correction[group_id]= 1.14594
exp_ipass= 0
sampling.NrDirections(0, true)= 48 sampling.NrDirections(0, false)= 16
sampling.NrPsiSamplings(0, true)= 96 sampling.NrPsiSamplings(0, false)= 6
mymodel.sigma2_noise[exp_ipart]=
I tried various ways to group the particles but always ran into
similar problems. Should I make the groups bigger, say 40 or 50 particle
images per group or even more? Or what is wrong with group 61?
Thanks a lot, best, Dieter
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Dieter Blaas,
Max F. Perutz Laboratories
Medical University of Vienna,
Inst. Med. Biochem., Vienna Biocenter (VBC),
Dr. Bohr Gasse 9/3,
A-1030 Vienna, Austria,
Tel: 0043 1 4277 61630,
Fax: 0043 1 4277 9616,
e-mail: [log in to unmask]
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