Hi Tofayel,
Something must be wrong with your mrc conversion. Perhaps you could try
another conversion program?
HTH, S
> Dear Dr. Scheres,
>
> This is Tofayel Ahmed, a first year graduate student in NTU Singapore. I
> am new to Cryo Electron Microscopy and currently learning to use it for my
> research. I find Relion to be a great tool to work with but I'm stuck due
> to some problem while using it. I converted the .raw files obtained from
> microscope using IMOD command : "raw2mrc -o 49 -x 4096 -y 4096 -t long -c
> IntermediateImage_yyyymmdd_xxxxxx_n*.raw" and then tried to use Relion to
> do further processing. While I could successfully run Relion and reproduce
> desired results using the test data set posted in relion tutorial webpage,
> I am unable to run it on my own image files. For your convenience, I have
> attached the snapshot of my shell terminal. It would be great if you could
> kindly go through it and suggest me some possible solutions ! Thank you
> very much for your time.
>
> Best regards,
> Tofayel
> NTU Singapore?
>
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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