Hi Dean,
Assuming you're meaning a 3D 1H-1H NOESY with combined 13C and 15N
editing/evolution in the third dimension, the simplest way is to load two
copies of the experiment, one with 13C referencing and the other with 15N
referencing.
Brian Smith
On Fri, 21 Feb 2014, Strotz von Moos Dean wrote:
> Hi everyone,
>
> I was wondering it it would be possible to design the experiment prototype NOESY -HSQC 13C/15N evolution.
> First of all it would be nice to have the distances in the Assignment window displayed for this type of experiment.
>
>
> But more importantly, I was wondering if it is possible to circumvent the internal resonance reference system.
>
> This is what I mean specifically; Obviously it is not possible to process the experiment simultaneously referenced to 13C and 15N. So what we usually do is reference it once to 13C and once to 15N and then assign either the 13C or 15N depending on how it was processed.
>
> However, in ccpnmr if one processed the experiment referenced to, lets say 13C, then one can not assign the 15N shifts since the resonance reference system prevents one from doing so.
>
> For the subsequent processing of our Data, it does however not matter if the 15N shifts are referenced correctly or not, they just have to be assigned.
>
> Any ideas on how to deal with this?
>
> Most sincerely
>
> Dean
>
--
Dr. Brian O. Smith --------------------------- Brian Smith at glasgow ac uk
Institute of Molecular, Cell and Systems Biology & School of Life Sciences,
College of Medical, Veterinary & Life Sciences,
Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, UK.
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