Never mind. Closing and reopening the software fixed the distance display issue in the F2 dimension.
However, if someone has an idea how to change the resonance assignment check for such a case that would be great. 13C and 15N in the same dimension, the third, would be great! It would safe us serious work hours. In our group at least three people regularly assign such spectra.
Many thanks and sincerely
Dean
On Feb 21, 2014, at 1:27 PM, Strotz von Moos Dean wrote:
> Hi,
>
> With respect to using NmrExpPrototype 27, there is the following problem as well;
> When setting the "Transfer Type Between Dims" to:
> 1 (1H) trough-space 2 (1H)
> 1 (1H) onebond 3 (13C)
>
> I do not get any distance information in the assignment window for F2, even if F1 and F3 are assigned. Instead I get only distance information in F1 if F2 is assigned (corraletd Dims is unchecked).
> What do I do wrong here?
>
> My thinking is that, with these settings, when F1 is assigned I should get the distance information for F2 (The structure is properly loaded, since the information is displayed in F1, when corraletd Dims is unchecked; with corraletd Dims checked I get now distance info)
>
> I am used to work with 2D NOESY and ccpNMR, so this is a bit of surprise.
>
> Greetings
>
> Dean
>
>
> On Feb 21, 2014, at 10:53 AM, Strotz von Moos Dean wrote:
>
>> Good morning Rasmus,
>>
>> That option I also explored, but even then: Assignment failed; Cannot assign resonances in C dimension to N atoms.
>>
>> Sincerely
>>
>> Dean
>>
>> On Feb 21, 2014, at 10:41 AM, Rasmus Fogh wrote:
>>
>>> Dear Dean,
>>>
>>> We do have the experiment prototype for that, so that cannot be the problem. It is NmrExpPrototype 27, systematic name 'H[{N|C}]_H.through-space', synonym 'HSQC-NOESY 15N/13C'. Have you tried using that? When you do, what are the problems you encounter?
>>>
>>> We could not decouple the resonance machinery - it is too fundamental. If there are some quite minor fixes that would help you we might have a look, but we are extremely busy with work on version 3 at the moment. If nothing else, Brian's idea of importing the same experiment once as 15N and once as 13C would be a good fix - you do not need to duplicate the data on disk.
>>>
>>> Please let us know what you come up with,
>>>
>>> Rasmus
>>>
>>>
>>> On 21/02/14 08:31, Strotz von Moos Dean wrote:
>>>> Hi everyone,
>>>>
>>>> I was wondering it it would be possible to design the experiment prototype NOESY -HSQC 13C/15N evolution.
>>>> First of all it would be nice to have the distances in the Assignment window displayed for this type of experiment.
>>>>
>>>>
>>>> But more importantly, I was wondering if it is possible to circumvent the internal resonance reference system.
>>>>
>>>> This is what I mean specifically; Obviously it is not possible to process the experiment simultaneously referenced to 13C and 15N. So what we usually do is reference it once to 13C and once to 15N and then assign either the 13C or 15N depending on how it was processed.
>>>>
>>>> However, in ccpnmr if one processed the experiment referenced to, lets say 13C, then one can not assign the 15N shifts since the resonance reference system prevents one from doing so.
>>>>
>>>> For the subsequent processing of our Data, it does however not matter if the 15N shifts are referenced correctly or not, they just have to be assigned.
>>>>
>>>> Any ideas on how to deal with this?
>>>>
>>>> Most sincerely
>>>>
>>>> Dean
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