Or if the tip is hopelessly pitted you can get a new one (for a Branson 1/2 inch horn) at:
http://www.proequip.com/productlist.asp?pcid=31
(thanks to:
http://blogs.cornell.edu/collinslab/2010/04/12/time-for-a-new-tip/ )
Reza Khayat wrote:
> A lot of "ageing" sonicators are not really ageing. Have you tried cleaning the tip of the sonicator? If not, remove the tip from the sonicator and use a 800 Grit wet sandpaper to make it as shiny as it was when you first bought it. I do this every three times I use the sonicator. A lot of crap deposits on it with every use.
>
> Best wishes,
> Reza
>
> Reza Khayat, PhD
> Assistant Professor
> The City College of New York
> Department of Chemistry, MR-1135
> 160 Convent Avenue
> New York, NY 10031
> Tel. (212) 650-6070
>
>
> ---- Original message ----
>> Date: Tue, 4 Feb 2014 12:40:51 -0500
>> From: CCP4 bulletin board <[log in to unmask]> (on behalf of Roger Rowlett <[log in to unmask]>)
>> Subject: Re: [ccp4bb] off-topic: bug busting
>> To: [log in to unmask]
>>
>> BeadBeater. http://biospec.com/. Gentle,
>> aerosol-free way to break 15-350 mL of cell paste
>> (2-150 g wet packed cells).
>>
>> Cheers,
>>
>> _______________________________________
>> Roger S. Rowlett
>> Gordon & Dorothy Kline Professor
>> Department of Chemistry
>> Colgate University
>> 13 Oak Drive
>> Hamilton, NY 13346
>>
>> tel: (315)-228-7245
>> ofc: (315)-228-7395
>> fax: (315)-228-7935
>> email: [log in to unmask]
>>
>> On 2/4/2014 11:49 AM, Phoebe A. Rice wrote:
>>
>> Some time ago, there was a nice discussion of
>> cost-effective, wimpy protein-friendly ways to
>> break open E. coli. We're thinking about
>> replacing an aging sonicator. If people have a
>> favorite gizmo, could they repeat that advice?
>> thank you,
>> Phoebe Rice
>>
>> ++++++++++++++++++++++++++++++++++++++++++
>>
>> Phoebe A. Rice
>> Dept. of Biochemistry & Molecular Biology
>> The University of Chicago
>>
>> 773 834 1723; [log in to unmask]
>> http://bmb.bsd.uchicago.edu/Faculty_and_Research/
>>
>> http://www.rsc.org/shop/books/2008/9780854042722.asp
>
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