Govindjee and Fork's biography of Stacey French is available here:
http://www.nasonline.org/publications/biographical-memoirs/memoir-pdfs/french-c-stacy.pdf
or if your library subscribes to photosynthesis research,
http://link.springer.com/article/10.1007%2Fs11099-006-0041-6
Mark J van Raaij wrote:
> LOL.
>
> In any case, a French press is also not french, according to Wikipedia it was invented by Charles Stacy French of the Carnegie Institution of Washington (hence French press with capital F).
>
> In any case, I agree, Emulsiflex, Constant Systems One-shot, Microfluidizer etc. is the way to go if you have some money.
>
> Mark J van Raaij
> Lab 20B
> Dpto de Estructura de Macromoleculas
> Centro Nacional de Biotecnologia - CSIC
> c/Darwin 3
> E-28049 Madrid, Spain
> tel. (+34) 91 585 4616
> http://www.cnb.csic.es/~mjvanraaij
>
>
>
>
>
> On 4 Feb 2014, at 18:09, Pascal Egea wrote:
>
>> Hi Phoebe,
>>
>> Another possibility is the Emulsiflex (from Avestin, in canada). Not a cheap piece of equipment, but very sturdy and efficient to up to 200 mL of extract (runs on house-air). Can deal with E coli (and even yeast if you have the models with internal compressor). It comes in 3 sizes I think we have the middle one (C-3) with a compressor. It is basically a french press but without the inconvenient of being french (I am french myself). It is quite gentle, and does not overheat samples as much as the sonicator. We make a lot of membrane protein purifications and I have been working with this since my post-doc
>> Hope this helps.
>>
>> Best regards,
>>
>> Pascal Egea
>>
>>
>> On Tue, Feb 4, 2014 at 8:49 AM, Phoebe A. Rice <[log in to unmask]> wrote:
>> Some time ago, there was a nice discussion of cost-effective, wimpy protein-friendly ways to break open E. coli. We're thinking about replacing an aging sonicator. If people have a favorite gizmo, could they repeat that advice?
>> thank you,
>> Phoebe Rice
>>
>> ++++++++++++++++++++++++++++++++++++++++++
>>
>> Phoebe A. Rice
>> Dept. of Biochemistry & Molecular Biology
>> The University of Chicago
>>
>> 773 834 1723; [log in to unmask]
>> http://bmb.bsd.uchicago.edu/Faculty_and_Research/
>>
>> http://www.rsc.org/shop/books/2008/9780854042722.asp
>>
>>
>>
>> --
>> Pascal F. Egea, PhD
>> Assistant Professor
>> UCLA, David Geffen School of Medicine
>> Department of Biological Chemistry
>> Boyer Hall room 356
>> 611 Charles E Young Drive East
>> Los Angeles CA 90095
>> office (310)-983-3515
>> lab (310)-983-3516
>> email [log in to unmask]
>
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