Adding 1-10mM copper sulfate is often a good way to oxidize disulfide bonds, although some proteins cannot tolerate this treatment.
Mike
----- Original Message -----
From: "Jacob Keller" <[log in to unmask]>
To: [log in to unmask]
Sent: Thursday, February 28, 2013 3:09:18 AM GMT -08:00 US/Canada Pacific
Subject: Re: [ccp4bb] disulfide engineering
Along these lines, what reagents do people use to promote disuflide bonds, i.e., the "anti-DTT?"
JPK
On Thu, Feb 28, 2013 at 2:06 AM, David Briggs < [log in to unmask] > wrote:
You might want to try "Disulfide by design"
http://cptweb.cpt.wayne.edu/DbD2/
Cheers
Dave
On Feb 28, 2013 6:55 AM, "Careina Edgooms" < [log in to unmask] > wrote:
Dear CCP4 members
I wish to engineer a disulfide bond at the dimer interface of a protein I am working with. Does anyone know of any available software to assist with this?
Best
Careina
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*******************************************
Jacob Pearson Keller, PhD
Postdoctoral Associate
HHMI Janelia Farms Research Campus
email: [log in to unmask]
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Michael C. Thompson
Graduate Student
Biochemistry & Molecular Biology Division
Department of Chemistry & Biochemistry
University of California, Los Angeles
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