I have used a copper solution that worked well. The details can be found in this paper: Nucleic Acids Res. 2004 Sep 30;32(17):5192-7. PMID: 15459288 PMCID: PMC521666
Yingyun Liu
----- Original Message -----
From: Roger Rowlett <[log in to unmask]>
Date: Thursday, February 28, 2013 9:52 am
Subject: Re: [ccp4bb] disulfide engineering
To: [log in to unmask]
> In the literature, you can find examples of air oxidation, oxidized
> glutathione (alone), mixture of reduced and oxidized glutathione, and
> hydrogen peroxide. The correct concentrations have to be found
> empirically. We are just now mushing through this with an engineered
> disulfide variant. Air oxidation partially worked for us--one of the
> engineered disulfides worked, one did not.
>
> _______________________________________
> Roger S. Rowlett
> Gordon & Dorothy Kline Professor
> Department of Chemistry
> Colgate University
> 13 Oak Drive
> Hamilton, NY 13346
>
> tel: (315)-228-7245
> ofc: (315)-228-7395
> fax: (315)-228-7935
> email: [log in to unmask]
>
> On 2/28/2013 7:55 AM, Clemens Grimm wrote:
> >>Along these lines, what reagents do people use to promote disuflide
> bonds,
> >>i.e., the "anti-DTT?"
> >
> >Glutathione (red) + Glutathione (ox), redox potential is adjusted by
> varying the ratio.
> >
> >Best,
> >Clemens
> >
> >
> >>
> >>JPK
> >>
> >>On Thu, Feb 28, 2013 at 2:06 AM, David Briggs <[log in to unmask]>wrote:
> >>
> >>>You might want to try "Disulfide by design"
> >>>
> >>>
> >>>
> >>>Cheers
> >>>
> >>>Dave
> >>>On Feb 28, 2013 6:55 AM, "Careina Edgooms" <[log in to unmask]>
> >>>wrote:
> >>>
> >>>>Dear CCP4 members
> >>>>
> >>>>I wish to engineer a disulfide bond at the dimer interface of a
> protein I
> >>>>am working with. Does anyone know of any available software to
> assist with
> >>>>this?
> >>>>
> >>>>Best
> >>>>Careina
> >>>>
> >>>
> >>
> >>
> >>--
> >>*******************************************
> >>Jacob Pearson Keller, PhD
> >>Postdoctoral Associate
> >>HHMI Janelia Farms Research Campus
> >>email: [log in to unmask]
> >>*******************************************
> >>
> >
> >
> >
> >--------------------------------------------------
> >Dr. Clemens Grimm
> >Institut für Biochemie
> >Biozentrum der Universität Würzburg
> >Am Hubland
> >D-97074 Würzburg
> >Germany
> >e-mail: [log in to unmask]
> >phone : +49 0931 31 84031
> >-------------------------------------------------
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