On 09/07/2010 02:52 PM, Marcel Jurk wrote:
> Dear all,
>
> During an ordinary assignment of my spectra I happened to discover that
> one spectrum had "magically" multiplied. I have no idea what caused this
> and/or where I might have clicked wrong. Did anyone have the same
> problem, yet? There is also no error message in the terminal.
> Since this could be quite unbelievable I attached a screen shot. You see
> an hncacb spectrum which should only have resonances (and which it did
> until this morning) between about 75 and 17 ppm. The spectrum seems
> copied somehow down to the carbonyl-region.
> Unfortunately, I saved and backuped the project before I realised this.
> When I open the spectrum in a new project it looks as did the days before.
>
> I can live with it but it would be nice if I could return everything
> back to normal.
>
> Best wishes and many thanks in advance for any helpful or critical advice,
> Marcel
>
>
>
Hi Marcel
this is an old cookie
when you have more than one spectrum shown in a window and multiple peak
lists enabled the peak picker will try to add a peak to all the spectra.
However, if some the spectra don't have un-aliased spectral intensity in
the region you picked it will enlarge the spectral bounds and show
aliased copies of the spectrum so you can see where the new peak picked
peak on top of spectra intensity so
1. at all costs avoid having multiple peak lists selected when manually
peak picking unless you want to pick in all the spectra
2. to get rid of the aliased spectra go to spectra-> referencing and
reset the minimum and maximum aliased frequencies to be clear which will
reset the aliased spectra limits to those in the original data file (ie
unaliased)
regards
gary
nb to tim there was a suggestion that analysis should ask about aliasing
the spectrum the first time a peak pick enlarges the bounds is this
possible to implement as it would save many users a lot of problems
regards
gary
--
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Dr Gary Thompson [Homans Lab Research Coordinator]
Astbury Centre for Structural Molecular Biology,
University of Leeds,
Leeds, LS2 9JT, West-Yorkshire, UK Tel. +44-113-3433024
email: [log in to unmask] Fax +44-113-3431935
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