Hari,
I have expressed sulfite oxidase (with a N-terminal heme) in E. coli strain TP1000 which resulted in red protein. I did not supplement the media with iron or a porphyrin precursor.
Sincerely,
James
On Aug 26, 2010, at 12:56 PM, Hari Namboodiri wrote:
> Hi CCPers
>
> Can anyone provide insights about expressing heme containing proteins in E.col? Does E.coli need any porphyrin precursor during expression or you need special E.coli strains. I have references mentioning delta-aminolevulinic acid for one ortholog but none for another. The enzyme is CYP51.
>
>
> Thanks
> Hari
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