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Hari, I have expressed sulfite oxidase (with a N-terminal heme) in E. coli strain TP1000 which resulted in red protein. I did not supplement the media with iron or a porphyrin precursor. Sincerely, James On Aug 26, 2010, at 12:56 PM, Hari Namboodiri wrote: > Hi CCPers > > Can anyone provide insights about expressing heme containing proteins in E.col? Does E.coli need any porphyrin precursor during expression or you need special E.coli strains. I have references mentioning delta-aminolevulinic acid for one ortholog but none for another. The enzyme is CYP51. > > > Thanks > Hari