Dear all:
Recently,I have a problem about molecular replacement with
identity 30% model. There are two moleculars in AU.Through
self-rotation analyze,there are a two-fold axis in AU along X axis or
Z axis.Then I rotate and translate the model with CNS MR module.
several models have been searched according self-rotation criterion.I
build the model using Phenix and Arp/wArp,but there are no good
results.The best result is R/Rfree 0.47/0.48.Also,I do MR with Phaser
and Molrep.The Phaser indicate Z-scores 6.5 and no good
results,neither do the Molrep.I am very confused about several
questions:
1.Just 30% identity model can be used for MR model?What at
least identity should be acquired for MR?
2.Are there good program editing the model except Chainsaw?
3.How can I make sure my rotated and translated MR model is
right and can be used for next model building and refinement?
--
Best
Wishes!
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