孙建 wrote:
> Dear all:
> Recently,I have a problem about molecular replacement with
> identity 30% model. There are two moleculars in AU.Through
> self-rotation analyze,there are a two-fold axis in AU along X axis or
> Z axis.Then I rotate and translate the model with CNS MR module.
> several models have been searched according self-rotation criterion.I
> build the model using Phenix and Arp/wArp,but there are no good
> results.The best result is R/Rfree 0.47/0.48.Also,I do MR with Phaser
> and Molrep.The Phaser indicate Z-scores 6.5 and no good
> results,neither do the Molrep.I am very confused about several
> questions:
> 1.Just 30% identity model can be used for MR model?What at
> least identity should be acquired for MR?
>
Hard to say. 30 or more than 20 should be on the edge. I think it should
depends on quality of your data and your model.
> 2.Are there good program editing the model except Chainsaw?
>
You can build your model manually by seaman or coot . Alternative
choice is mumbo.
> 3.How can I make sure my rotated and translated MR model is
> right and can be used for next model building and refinement?
>
>
>
I don't know. I only test the solutions by refinement. The one ,which
can be refined , is the right one. Otherwise it is not.
Good luck,
leo
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