Would you put the iodide protocol on the wiki? If not, please send it to me
directly.
JPK
*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: [log in to unmask]
*******************************************
----- Original Message -----
From: <[log in to unmask]>
To: <[log in to unmask]>
Sent: Tuesday, March 31, 2009 1:07 PM
Subject: Re: [ccp4bb] Halide soaking
I'd like to remind folks here that if one's crystals are sturdy enough to
survive halide soaking, they're *probably* also sturdy enough to live
through covalent iodination. Iodination is easy to set up and if it does
work out - one gets awesome quality derivatives with multiple sites (as
long as there are enough Tyrosines in the protein). The drawback is that
iodination can and often does kill the crystal - which is the reason for
my earlier comment regarding crystal machismo/resilience.
If you want to try it, I would be happy to supply an easy protocol that
worked for me several times :)
Artem
> You always get the entry of Bromide into crystal by quick soaking,
> because it does not require the incorporation of Bromide into the
> protein. But whether the signal is good enough for phasing is another
> story. You have to collect the full data set to know the answer.
>
> Nian
>
> 2009/3/31 tat cheung cheng <[log in to unmask]>:
>> Hi all
>>
>> I am now trying to do bromide soaking, but i am not really sure does the
>> bromide atom enter my crystal. So is there any signs that indicate the
>> entry of bromide atom? e.g. does the space group, cell dimension change?
>> or just nothing change, and the bromide atom just get in?
>> Thanks very much.
>>
>> T.C. Cheng
>>
>>
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>
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