Hi,
it worked very nice for me in 1 out of 1 case where I tried it :-).
Very well diffracting crystals (1.8 Ang), rather small protein 20
kDa, 50 %solvent content, 1 mol/ASU. 20-30 s soak in 0.5 M NaBr
resulted in 6 nice ordered sites.
It was crucial for us to collect a 3 wavelength MAD data set. A SAD
data set (using just the peak, even if with high redundancy ) was not
enough to obtain traceable electron density map, even-though
one could distinguish clearly protein boundaries and solvent channels.
Good luck
Ale
On 31 Mar 2009, at 18:19, tat cheung cheng wrote:
> Hi all
>
> I am now trying to do bromide soaking, but i am not really sure does
> the bromide atom enter my crystal. So is there any signs that
> indicate the entry of bromide atom? e.g. does the space group, cell
> dimension change? or just nothing change, and the bromide atom just
> get in?
> Thanks very much.
>
> T.C. Cheng
>
>
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