You could use salts such as LiSO4 for cryo-protection (also amm sulphate
with small crystals has worked to a degree with us, when nothing else
worked, also 1.6-1.7 M amm. sulphate was exchangable to 40% PEG 400 (very
quick), although with not so great results...).
which oil did you try? that would make a lot of difference..
(see also the recent discussion on LN2/propane etc and how to freeze)
hth,
Tommi
Quoting Ji lee <[log in to unmask]>:
> Dear,
>
> I have a crystal diffracted anisotrophically. I tested with a few
> different cryo conditions like oil, glycerol in different
> concentration to get a better data but these conditions didn't help
> any.
> Using capillary method improved the diffraction (isotrophic) but the
> crystal couldn't survive during the data collection.
> Is there any methods or cryo conditions I can use to improve my
> diffraction data?
> This crystal grew in 2.5M Ammonium Sulfate.
>
> Thank you so much.
>
> Ji
>
>
--
Tommi Kajander, Ph.D.
Macromolecular X-ray Crystallography
Research Program in Structural Biology and Biophysics
Institute of Biotechnology
P.O. Box 65 (Street address: Viikinkaari 1, 4th floor)
University of Helsinki
FIN-00014 Helsinki, Finland
Tel. +358-9-191 58903
Fax +358-9-191 59940
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