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You could use salts such as LiSO4 for cryo-protection (also amm sulphate with small crystals has worked to a degree with us, when nothing else worked, also 1.6-1.7 M amm. sulphate was exchangable to 40% PEG 400 (very quick), although with not so great results...). which oil did you try? that would make a lot of difference.. (see also the recent discussion on LN2/propane etc and how to freeze) hth, Tommi Quoting Ji lee <[log in to unmask]>: > Dear, > > I have a crystal diffracted anisotrophically. I tested with a few > different cryo conditions like oil, glycerol in different > concentration to get a better data but these conditions didn't help > any. > Using capillary method improved the diffraction (isotrophic) but the > crystal couldn't survive during the data collection. > Is there any methods or cryo conditions I can use to improve my > diffraction data? > This crystal grew in 2.5M Ammonium Sulfate. > > Thank you so much. > > Ji > > -- Tommi Kajander, Ph.D. Macromolecular X-ray Crystallography Research Program in Structural Biology and Biophysics Institute of Biotechnology P.O. Box 65 (Street address: Viikinkaari 1, 4th floor) University of Helsinki FIN-00014 Helsinki, Finland Tel. +358-9-191 58903 Fax +358-9-191 59940