I don't have the quantitative data any more, but interferences in the
Biuret method depend on the concentration of protein at which you are
measuring and, at the concntrations found in serum, glucose is not a
significant interfering substance. Sucrose can cause interference if
present at high enough concentration (this would be from an ex-vivo
sucrose gradient, rather than a physiological situation). Lipids
interfere by causing turbidity and hence the recomended "long"
procedure of decolourising with KCN after the reaction and re-reading
the blank (I doubt that many people do that any more). Reference
Gornall AG et al J Bio,Chem 1949 177:751-766 if you want to read the
original paper.
Nick Miller
London
On 13/09/2007, Dr. Michael Steiner <[log in to unmask]> wrote:
> Dear Lab Community,
>
> Is the interference of hyperglycaemia on the Biuret protein assay a real
> thing or a "may be" textbook statement ? Any quantitative data (most
> probably from the preceding century ...)?
>
> This question came up while discussing total protein methodology.
>
> Thank you for your input.
>
> Mike
>
> Michael Steiner
> Institute of Clinical Chemistry & Laboratory Medicine
> University of Rostock
> D-18057 Rostock
> GERMANY
>
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