Stephen, We recently audited the Roche prolactin II assay. Out of 224
individuals over 11 months with prolactin >700 mIU/l, in-house PEG
precipitation screening showed 4 were macroprolactin positive (<40%
recovery) and 7 were equivocal (40-60% recovery). Of the latter that we
referred to Southend for confirmation, 4 were positive, 2 remained
equivocal and 1 was negative. We continue to screen all samples where
prolactin >700 by Roche prolactin II unless there has already been a
screen result within the previous 12 months. Hope this helps.
Anne
Dr Anne Dawnay PhD FRCPath
Consultant Biochemist
University College London Hospitals
Clinical Biochemistry
60 Whitfield St
London W1T 4EU
Tel +44 (0)8451555000 ext 2954
Fax +44 (0)20 7380 9584
email [log in to unmask]
Date: Thu, 3 May 2007 15:15:03 +0100
From: "Frost, Stephen" <[log in to unmask]>
Subject: macroprolactin
The current Roche Modular prolactin kit is more specific for monomeric
prolactin and consequently now only has occasional significant
interference by macroprolactin, compared to the previous formulation in
which it was quite common. We are still checking high samples by the PEG
precipitation technique. I wondered if any Modular users are still
seeing a significant number of macroprolactinaemia samples with the
current kit. If not have any of you been brave enough to stop checking
high prolactin samples for macroprolactin?
Regards
Steve
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