Hi,
I dare say with an R-factor of 0.64, your MR solution is simply wrong.
Just into the blue, you could
1) try various MR-programs (phaser, molrep, amore...)
2) try a different search model
3) try your best model but start with subdomains or chop off possibly
flexible parts
4) have a look at the CaspR server
http://www.igs.cnrs-mrs.fr/Caspr2/index.cgi
5) with crystals that give such good data try to get other pahse
information (soaks, co-crystallisation, SAD (SAD at home), etc.)
Good luck, Tim
--
Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
GPG Key ID = A46BEE1A
On Fri, 16 Feb 2007, Emmanuel Prata wrote:
> Dear all,
>
> I have a data set of a protein soaked with sacarose, and the statistic
> data is good until the molecular replacement (Rmerge 0,07 (24%),
> I/Sigma 7 (2,7), completness (99,8 (98%)), and resolution 1,98A. After
> this step, I can not get the Rfactor (64%) to drop (I believe the
> spacegroup is correct).
> Could anyone give me suggestions?
> Thank you in advance,
> Emmanuel Prata
>
|