Hi Shivesh,
Have you tried dropping your MPD concentration (ie. by increments of
~5%)? Changing the pH may also result in larger crystals.
JP
On 1/30/07, shivesh kumar <[log in to unmask]> wrote:
> Dear all
> Sorry for unrelated query.I have crystallized a protein which is at 40% of
> MPD.But the problem is that I get several crystals but these are
> small.Thecrystals diffract upto
> 3A.I welcome any suggestion to make the crystals big enough to diffract at
> high resolution.What additives I can add???Thanx in advance.
> Shivesh
>
>
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