Sorry to be late in responding to this query for "old papers" but I have
been on holiday. A mixture of cetrimide and fluoride at pH 4.0 (Long's
mixture) was the first to describe the stabilization of blood pyruvate
without the use of trichloroacetic acid precipitation (Long C, Biochem
J 1944; 38: 447). It was subsequently shown to work for lactate too
(Powell FJN, Clin Chim Acta 1974; 55: 107) . Hope these are old enough
references for you!!
Brian Payne
Dr. M. Steiner wrote:
>Dear colleagues,
>
>We have got a request to run several hundreds of pyruvate analyses for
>some "Biochemistry of exercise" project.
>I fully realise that pyruvate is extremely unstable in blood. What I
>wonder about is if we indeed will need to use some acid to immediately
>precipitate proteins. This might be hard to accomplish for logistic
>reasons. Would fluoride-containing blood tubes be an alternative with
>the idea to stop lactate generation (and stabilize pyruvate levels,
>too)? Still, I am not sure if pyruvate analysis will be reliable under
>these conditions?
>I am sure there are "old papers" on this which I have never seen nor
>read ...
>
>Thank you very much for your input.
>
>Dr. med. Michael Steiner
>University of Rostock
>Institute of Clinical Chemistry & Pathobiochemistry
>D-18057 Rostock
>GERMANY
>
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