Many years ago (>25), we used amniotic fluid as "positive" control for
CK-BB isoenzymes (electrophoresis method)
think that this can still be performed with several kits for immunoassays
(e.g., Roche, etc).
There is a lot of CK-BB activity in amniotic fluid but none in urine.
"Reminiscence is the essence of senescence"
AOV
On Wed, 30 Oct 2002, Everitt, Tony wrote:
> Sue
>
> I wondered too, whether the POC fetal fibronectin test might help. The test
> appears to have good 'rule-out' for risk of pre-term delivery in the next
> week. As well as the web-site (www.ffntest.com) there is also a Bandolier
> article http://www.jr2.ox.ac.uk/bandolier/band43/b43-2.html.
>
> Rgds
>
> Tony Everitt
> BAsildon Hospital
>
> -----Original Message-----
> From: p=NHS NATIONAL
> INT;a=NHS;c=GB;dda:RFC-822=ACB-CLIN-CHEM-GEN(a)JISCMAIL.AC.UK;
> Sent: 29 October 2002 17:35
> To: p=NHS NATIONAL
> INT;a=NHS;c=GB;dda:RFC-822=ACB-CLIN-CHEM-GEN(a)JISCMAIL.AC.UK;
> Subject: Discriminating amniotic fluid from urine in real time for ?PROM
>
>
> An obstetrician has just enquired if there is a quick reliable biochemical
> (or other) test that could be used in possible PROM (premature rupture of
> membrane) cases. They had a lady who came in with a container of fluid, she
> thought she had PROM at 23/40 but there was no obvious liquid in the
> vagina. They thought it probably was PROM and tried an induction which
> failed and she later delivered normally at 38/40. We don't do AFP on site
> so I'm looking for something routinely available - quick/easy/RELIABLE to
> distinguish amniotic fluid from urine. TP? creat? (fetal urine contributes
> how much to amniotic fluid, depending on gestation?) HELP please. Looked on
> internet but nothing sprang out at me. Any suggestions gratefully received.
> THanks, Sue Walker, Salisbury UK.
>
> ------ACB discussion List Information--------
> This is an open discussion list for the academic and clinical
> community working in clinical biochemistry.
> Please note, archived messages are public and can be viewed
> via the internet. Views expressed are those of the individual and
> they are responsible for all message content.
>
> ACB Web Site
> http://www.acb.org.uk
> List Archives
> http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html
> List Instructions (How to leave etc.)
> http://www.jiscmail.ac.uk/
>
>
> **********************************************************************
> This email and any files transmitted with it are confidential and
> intended solely for the use of the individual or entity to whom they
> are addressed. If you have received this email in error please notify
> the system manager at mailto:[log in to unmask]
>
> This footnote also confirms that this email message has been swept by
> MIMEsweeper for the presence of computer viruses.
>
> www.mimesweeper.com
> **********************************************************************
>
> ------ACB discussion List Information--------
> This is an open discussion list for the academic and clinical
> community working in clinical biochemistry.
> Please note, archived messages are public and can be viewed
> via the internet. Views expressed are those of the individual and
> they are responsible for all message content.
>
> ACB Web Site
> http://www.acb.org.uk
> List Archives
> http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html
> List Instructions (How to leave etc.)
> http://www.jiscmail.ac.uk/
>
>
------ACB discussion List Information--------
This is an open discussion list for the academic and clinical
community working in clinical biochemistry.
Please note, archived messages are public and can be viewed
via the internet. Views expressed are those of the individual and
they are responsible for all message content.
ACB Web Site
http://www.acb.org.uk
List Archives
http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html
List Instructions (How to leave etc.)
http://www.jiscmail.ac.uk/
|