We do electrophoresis on a sample stored at 37 C to check for monoclonal
cryoglobulinaemia (Type 1). We then do immunoglobulins on samples stored at
both 4 C (for 48 hrs) and 37 C and compare the results.
Cryoglobulins are reported as positive if results differ significantly and
there is visual evidence of a cryoglobulin.
Ian Barlow
> -----Original Message-----
> From: Graham Bishell [SMTP:[log in to unmask]]
> Sent: Tuesday, April 11, 2000 10:28 PM
> To: [log in to unmask]
> Subject: Cryoglobulins
>
> Methods for the detection of cryoglobulin are not particularly advanced.
> We have been looking at trying to make the method less subjective by
> measuring absorbance change upon warming up serum/plasma, with some
> success. Has anyone used a similar or other approach to reducing the
> subjectivity of cryoglobulin detection.
> P.S. If a sample is stored at 4 degrees C and mixed before examination,
> we have noticed that chylomicrons appear to give a turbidity to the
> specimen that disappears on re-heating. In extreme cases it would be
> possible for this to cause false positive results for cryoglobulin with
> inexperienced analysts.
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