We adopted the advice of Abbott to run AxSym oestradiols on a 1 in 5
dilution, repeating those <551pmol/L neat (as described in device correction
B7A639, July 2000). We assayed controls neat, as instructed.
Recent poor external QC performance (with many samples run on dilution)
prompted a brief review of this practice. This seems to show: a)very poor
precision for diluted samples and b)often significantly higher results on
diluted samples (compared to neat.)
Has anyone else done similar checks and what was found? It could be just
our Instrument, if others have good results.
Please reply to me directly, or to the mailbase.
Thanks
Graham Ellis
St. John's Hospital
Livingston
West Lothian
Scotland
01506 422756
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